基于质谱的蛋白质从头测序在抗体、新抗原、未知物种蛋白测序中有重要应用。现有从头测序技术受制于质谱信息的缺失，测序覆盖度和准确性较低。中科院数学院与合作单位基于镜像酶实验策略和多个创新性人工智能及统计方法研发了自主知识产权的蛋白质从头测序软件系统DiNovo，将高可靠序列覆盖度提高了两倍，并在国内多家单位成功试用。相关成果发表在《自然通讯》（Nature Communications）上，并获得专利授权和软件著作权。

Publication:

Nature Communications

https://doi.org/10.1038/s41467-026-70224-6

Author:

Zixuan Cao

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

These authors contributed equally: Zixuan Cao, Xueli Peng, Di Zhang, Piyu Zhou, Li Kang.

Xueli Peng

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

These authors contributed equally: Zixuan Cao, Xueli Peng, Di Zhang, Piyu Zhou, Li Kang.

Di Zhang

School of Computer Science and Technology, Shandong University of Technology, Zibo, China.

These authors contributed equally: Zixuan Cao, Xueli Peng, Di Zhang, Piyu Zhou, Li Kang.

Piyu Zhou

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

These authors contributed equally: Zixuan Cao, Xueli Peng, Di Zhang, Piyu Zhou, Li Kang.

Li Kang

State Key Laboratory ofMedical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics and Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing, China. 

Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, China. 

These authors contributed equally: Zixuan Cao, Xueli Peng, Di Zhang, Piyu Zhou, Li Kang.

Hao Chi

University of Chinese Academy of Sciences, Beijing, China.

Key Laboratory of Intelligent Information Processing of Chinese Academy of Sciences, Institute of Computing Technology, Chinese Academy of Sciences, Beijing, China. 

Ruitao Wu

School of Computer Science and Technology, Shandong University of Technology, Zibo, China.

Zhiyuan Cheng

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

Yao Zhang

State Key Laboratory ofMedical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics and Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing, China.

Jiaxing Dai

State Key Laboratory ofMedical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics and Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing, China.

Yanchang Li

State Key Laboratory ofMedical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics and Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing, China.

Lijin Yao

School of Computer Science and Technology, Shandong University of Technology, Zibo, China.

Xinming Li

School of Computer Science and Technology, Shandong University of Technology, Zibo, China.

Yaoyu He

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

Jinghan Yang

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China.

Haipeng Wang

School of Computer Science and Technology, Shandong University of Technology, Zibo, China. 

e-mail: hpwang@sdut.edu.cn

Ping Xu

State Key Laboratory ofMedical Proteomics, National Center for Protein Sciences (Beijing), Research Unit of Proteomics and Research and Development of New Drug of Chinese Academy of Medical Sciences, Beijing Proteome Research Center, Beijing Institute of Lifeomics, Beijing, China. 

Program of Environmental Toxicology, School of Public Health, China Medical University, Shenyang, China.

e-mail: xuping_bprc@126.com

 Yan Fu

State Key Laboratory of Mathematical Science, Academy of Mathematics and Systems Science, Chinese Academy of Sciences, Beijing, China. 

University of Chinese Academy of Sciences, Beijing, China. 

e-mail: yfu@amss.ac.cn